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Feb 13 2018

Nonribosomal Peptide and Polyketide Biosynthesis – Methods, Bradley S, peptide biosynthesis.#Peptide #biosynthesis


Nonribosomal Peptide and Polyketide Biosynthesis

  • Includes cutting-edge methods and protocols for NPRS and PKS research
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

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  • ISBN 978-1-4939-3375-4
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase

Hardcover $119.00

This volume presents an overview of nonribosomal peptide synthetase (NRPS) and polyketide synthase (PKS) structure and function. It then continues with methods for the analysis of these pathways including conventional enzymological assays, contemporary mass spectrometric analysis techniques, specialized molecular biological approaches applicable to NRPSs and PKSs, and small molecule analysis tools tailored to this very special class of natural products, and concludes by examining bioinformatics tools for the analysis of these enzymes, pathways, and molecules. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Nonribosomal Peptide and Polyketide Biosynthesis: Methods and Protocols serves as a valuable reference for those experienced in studying NRPS and PKS enzymes, pathways, and natural products as well as a gateway for those just entering the field.

Table of contents (17 chapters)

Structural Biology of Nonribosomal Peptide Synthetases

Miller, Bradley R. (et al.)

The Assembly Line Enzymology of Polyketide Biosynthesis

Till, Marisa (et al.)

Measurement of Nonribosomal Peptide Synthetase Adenylation Domain Activity Using a Continuous Hydroxylamine Release Assay

Duckworth, Benjamin P. (et al.)

Affinity Purification Method for the Identification of Nonribosomal Peptide Biosynthetic Enzymes Using a Synthetic Probe for Adenylation Domains

Ishikawa, Fumihiro (et al.)

Colorimetric Detection of the Adenylation Activity in Nonribosomal Peptide Synthetases

Maruyama, Chitose (et al.)

Facile Synthetic Access to Glycopeptide Antibiotic Precursor Peptides for the Investigation of Cytochrome P450 Action in Glycopeptide Antibiotic Biosynthesis

Brieke, Clara (et al.)

Reconstitution of Fungal Nonribosomal Peptide Synthetases in Yeast and In Vitro

Cacho, Ralph A. (et al.)

The Continuing Development of E. coli as a Heterologous Host for Complex Natural Product Biosynthesis

Zhang, Haoran (et al.)

Screening for Expressed Nonribosomal Peptide Synthetases and Polyketide Synthases Using LC-MS/MS-Based Proteomics

Chen, Yunqiu (et al.)

Enhancing Nonribosomal Peptide Biosynthesis in Filamentous Fungi

Soukup, Alexandra A. (et al.)

In Situ Analysis of Bacterial Lipopeptide Antibiotics by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging

Debois, Delphine (et al.)

Secondary Metabolic Pathway-Targeted Metabolomics

Vizcaino, Maria I. (et al.)

Annotating and Interpreting Linear and Cyclic Peptide Tandem Mass Spectra

Niedermeyer, Timo Horst Johannes

Bioinformatics Tools for the Discovery of New Nonribosomal Peptides

Leclère, Valérie (et al.)

The Use of ClusterMine360 for the Analysis of Polyketide and Nonribosomal Peptide Biosynthetic Pathways

Tremblay, Nicolas (et al.)

Alignment-Free Methods for the Detection and Specificity Prediction of Adenylation Domains

Agüero-Chapin, Guillermin (et al.)

Characterization of Nonribosomal Peptide Synthetases with NRPSsp

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